Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays
- 14 June 2019
- book chapter
- Published by Springer Nature
- Vol. 2017, 193-203
Abstract
Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.Keywords
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