Effect of Bordetella pertussis on α1and β-Adrenergic and Cholinergic Muscarinic Receptors in Guinea Pig Lung Membranes

Authors:
  • HMHaruhisa Mita
    Haruhisa Mita
  • YYYasuo Yui
    Yasuo Yui
  • MSMuneo Suzuki
    Muneo Suzuki
  • HYHiroshi Yasueda
    Hiroshi Yasueda
  • TSTakao Shida
    Takao Shida
Publisher website
Google Scholar
Abstract
After intraperitoneal injection of Bordetella pertussis vaccine to guinea pigs, the α1- and β-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, 3H-prazosin, 1-3H-dihydroalprenolol and 1-3H-quinuclidinyl benzilate, respectively. Injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (Bmax) of 3H-prazosin, while there was about a 30% reduction in 1-3H-dihydroalprenolol binding to β-adrenergic receptors. No difference was observed in the dissociation constant (KD) for binding of each ligand to α1- and β-adrenergic receptors. 1-3H-quinuclidinyl benzilate binding indicated that the Bmax and KD for cholinergic muscarinic receptor in pertussis-sensitized guinea pigs were not significantly different from those normal control animals.

This publication has 9 references indexed in Scilit: