Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC
Authors:
- Karlheinz MannKMKarlheinz Mann
- Rainer DeutzmannRDRainer Deutzmann
- Mats PaulssonMPMats Paulsson
- Rupert TimplRTRupert Timpl
- 22 June 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 218 (1) , 167-172
Abstract
Up to 80% of the calcium‐binding protein BM‐40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.Keywords
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