Re-analysis of mobile mRNA datasets raises questions about the extent of long-distance mRNA communication

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Abstract
Short-read RNA-seq studies of grafted plants have led to the proposal that thousands of messenger RNAs (mRNAs) move over long distances between plant tissues1–7, potentially acting as signals8–12. Transport of mRNAs between cells and tissues has been shown to play a role in several physiological and developmental processes in plants, such as tuberization13, leaf development14 and meristem maintenance15; yet for most mobile mRNAs, the biological relevance of transport remains to be determined16–19. Here we perform a meta-analysis of existing mobile mRNA datasets and examine the associated bioinformatic pipelines. Taking technological noise, biological variation, potential contamination and incomplete genome assemblies into account, we find that a high percentage of currently annotated graft-mobile transcripts are left without statistical support from available RNA-seq data. This meta-analysis challenges the findings of previous studies and current views on mRNA communication.
Funding Information
  • RCUK | Biotechnology and Biological Sciences Research Council (BB/X01102X/1, BB/X010996/1, BB/X007685/1, BB/X016056/1 and BB/Y008782/1., BB/X010996/1, BB/X007685/1, BB/X016056/1 and BB/Y008782/1.)
  • EC | Horizon 2020 Framework Programme (810131, 810131, 810131, 810131, 810131, 810131, 810131, 810131, 810131, 810131)
  • National Science Foundation (DBI-2019674, IOS-1942437, DGE-2139899, DGE-2139899, DBI-2019674, IOS-1942437)
  • Deutsche Forschungsgemeinschaft (433194101)

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