Suspension Array with Shape-Coded Silica Nanotubes for Multiplexed Immunoassays
Authors:
- 7 June 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (14) , 5257-5263
Abstract
A suspension array for multiplexed immunoassays has been developed using shape-coded silica nanotubes (SNTs) as coding materials. Fabricated by multistep anodization template synthesis, each shape-coded SNT has several segments with different reflectance values depending on their diameters and wall thicknesses. Therefore, the code of each SNT can be “read-out” under a conventional optical microscope. The suspension array with shape-coded SNTs has shown high stability and dispersibility in aqueous buffer media and high detection sensitivity. The SNTs have not shown any visible degradation while submerged in aqueous solution for 7 months, the tubular structure and silanol groups on the inner and outer surfaces allow SNTs to disperse evenly in buffer solution, and the detection limit of an IgG protein is about 6 pM with 1.5 × 106 SNTs per mL. We have demonstrated the high selectivity of the SNTs suspension array for the detection of multianalytes in the multiplexed immunoassay experiments.Keywords
This publication has 12 references indexed in Scilit:
- Qdot Nanobarcodes for Multiplexed Gene Expression AnalysisNano Letters, 2006
- Stabilization of Silver Metal in Citrate Buffer: Barcoded Nanowires and Their BioconjugatesChemistry of Materials, 2005
- Particle Formation by Ion Nucleation in the Upper Troposphere and Lower StratosphereScience, 2003
- Templated Surface Sol–Gel Synthesis of SiO2 Nanotubes and SiO2‐Insulated Metal NanowiresAdvanced Materials, 2003
- Striped Metal Nanowires as Building Blocks and Optical TagsAdvanced Materials, 2003
- The emerging field of nanotube biotechnologyNature Reviews Drug Discovery, 2003
- Protein microarray technologyTrends in Biotechnology, 2002
- Suspension array technology: evolution of the flat-array paradigmTrends in Biotechnology, 2002
- Solution and chip arrays in protein profilingTrends in Biotechnology, 2001
- Direct detection of nucleic acid hybridization on the surface of a charge coupled deviceNucleic Acids Research, 1994