Staphylococcus Aureus Surface Protein G is An Immunodominant Protein and a Possible Target in An Anti-Biofilm Drug Development

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Abstract
Background: Staphylococcus aureusis a Gram-positive bacterium that causes severe illnesses in the human population. The capacity ofS. aureusstrains to form biofilms on biotic and abiotic surfaces creates serious problems for treatment of hospital infections and has stimulated efforts to develop new means of specific protection or immunotherapy.Material and Methods: We found that rabbit serum raised against crude concentratedS. aureusliquid culture significantly decreased the development of staphylococcal biofilmin vitro. To discover the corresponding staphylococcal antigen, we used mass-spectrometry and molecular cloning and identified three major immunodominant proteins. They included α-haemolysin, serine proteinase SplB andS. aureussurface protein G, known as adhesin SasG.Results: Although according to literature data, all these proteins represent virulence factors ofS. aureusand play diverse and important roles in the pathogenesis of staphylococcal diseases, only SasG can be directly implicated into the biofilm formation because of its surface location on a staphylococcal cell. Indeed, rabbit serum directed against purified recombinant SasG, similar to serum against crude staphylococcal liquid culture, prevented the formation of a biofilm.Conclusion: SasG can be considered as a target in an anti-biofilm drug development and a component of the vaccine or immunotherapeutic preparations directed against staphylococcal infections in humans.

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