Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants
Authors:
- Bruce BudowleBBBruce Budowle
- Patricia EberhardtPEPatricia Eberhardt
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 10 (2) , 161-172
Abstract
An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.Keywords
This publication has 15 references indexed in Scilit:
- Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusingElectrophoresis, 1985
- Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gelsElectrophoresis, 1984
- Pseudo‐ligand affinity chromatography and high‐voltage isoelectric focusing as a multiparameter method for separation and identification of plasma proteinsElectrophoresis, 1983
- Neonatal screening of β‐thalassemias by thin layer isoelectric focusingAmerican Journal of Hematology, 1982
- Human globin chain separation by isoelectric focusing in ultrathin polyacrylamide gelsClinica Chimica Acta, 1980
- Ultrathin‐layer isoelectric focusing in 50–100 μm polyacrylamide gels on silanized glass plates or polyester filmsElectrophoresis, 1980
- THE USE OF GEL ELECTROFOCUSING IN THE STUDY OF HUMAN HEMOGLOBINS*Annals of the New York Academy of Sciences, 1973
- Thin-layer isoelectric focusing for haemoglobin screening and its application to haemoglobin MalmöClinica Chimica Acta, 1972
- Separation and characterization of a foetal haemoglobin variant by means of isoelectric focussingLife Sciences, 1971
- The separation of human and animal hemoglobins by isoelectric focusing in polyacrylamide gelBiochimica et Biophysica Acta (BBA) - Protein Structure, 1971