Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides
Authors:
- Rudolf Volkmer-EngertRVRudolf Volkmer-Engert
- Margit SchmidtMSMargit Schmidt
- Marco SchulzMSMarco Schulz
- Jens Schneider-MergenerJSJens Schneider-Mergener
- Ulrich ReinekeURUlrich Reineke
- 1 January 2000
- journal article
- review article
- Published by Wiley in Biopolymers
- Vol. 55 (3) , 188-206
Abstract
Since its invention the SPOT-synthesis methodology has become one of the most efficient strategies for the miniaturized assembly of large numbers of peptides. The combination of a facile synthetic method with high throughput solid- and solution-phase screening assays qualifies the SPOT-technique as a valuable tool in biomedical research. Recent developments such as the introduction of novel polymeric surfaces, new linker and cleavage strategies as well as automated robot systems extended the scope of practical chemical reactions that can be accommodated as well as the numbers of compounds obtainable by this technique. Thus, highly complex spatially addressed compound arrays have become accessible. Together with the introduction of novel screening assays, the method is excellently suited to elucidate recognition events on the molecular level.Keywords
This publication has 68 references indexed in Scilit:
- Selection of antibody probes to correlate protein sequence domains with their structural distributionProtein Science, 1999
- Use of immobilized synthetic peptides for the identification of contact sites between human interleukin‐6 and its receptorFEBS Letters, 1996
- Identification of Technetium-99m Binding Peptides Using Combinatorial Cellulose-Bound Peptide LibrariesJournal of the American Chemical Society, 1995
- Combinatorial Cellulose-Bound Peptide Libraries: Screening Tools for the Identification of Peptides That Bind Ligands with Predefined SpecificityMethods, 1994
- T cell epitope analysis with peptides simultaneously synthesized on cellulose membranes: Fine mapping of two DQ dependent epitopesFEBS Letters, 1994
- Structural base of the interaction of a monoclonal antibody against p24 of HIV-1 with its peptide epitopeMolecular Immunology, 1993
- Long-chain polystyrene-grafted polyethylene film matrix: a new support for solid-phase peptide synthesisJournal of the American Chemical Society, 1989
- Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicatorCollection of Czechoslovak Chemical Communications, 1988
- Improved approach for anchoring Nα‐9‐fluorenylmethyloxycarbonylamino acids as p‐alkoxybenzyl esters in solid‐phase peptide synthesisInternational Journal of Peptide and Protein Research, 1985
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963