Detection of IgM Antibodies to Cytomegalovirus (CMV) Using an Enzyme-labelled Antigen (ELA)
Authors:
- H. SchmitzHSH. Schmitz
- U. von DeimlingUDU. von Deimling
- 1 September 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 50 (1) , 59-68
Abstract
We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.Keywords
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