Does diphenylene iodonium chloride have any effect on the O2−‐generating step of plant peroxidases?
Authors:
- 30 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (3) , 254-256
Abstract
The O2 −‐generating step of plant peroxidases during their catalytic cycle is represented by the decay of compound III (CoIII) into ferriperoxidase, which most likely involves the dissociation of a ferric‐O2 − complex to yield the ferric form of the enzyme and O2 −. Diphenylene iodonium chloride (DPI), at 50–100 μM, does not significantly enhance the stability of CoIII of peroxidase, as judged by the values of k decay, and therefore, DPI appears to have no effect on the O2 −‐generating step of plant peroxidases. From these results, it is concluded that caution should be exercized when considering peroxidase as a possible enzyme target of O2 −‐mediated plant physiological processes sensitive to DPI inhibition.Keywords
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