Promoter recognition by phage SP01-modified RNA polymerase.
Authors:
- C TalkingtonCTC Talkington
- J PeroJPJ Pero
- 1 March 1978
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (3) , 1185-1189
Abstract
A modified form of Bacillus subtilis RNA polymerase containing a phage SP01-coded regulatory protein (the gene 28 product) selectively transcribes "middle" genes of the phage genome in vitro. In this paper, we identify a subset of restriction endonuclease fragments of SP01 DNA that promote specific transcription by the phage-modified polymerase. In the absence of nucleoside triphosphates, RNA polymerase containing the gene 28 protein selectively binds to these DNA fragments thereby forming stable binary complexes that can be isolated on nitrocellulose filters. In contrast, unmodified RNA polymerase containing sigma factor selectively binds to and transcribes a subset of phage DNA fragments that contain "early" sequences and that are in large part distinct from the fragments recognized by the phage-modified transcriptase. Our results strongly suggest that phage "early" and "middle" genes are transcribed from distinct promoters and that the RNA polymerase containing the gene 28 protein binds to sites that are located at or near promoters for SP01 "middle" genes.Keywords
This publication has 17 references indexed in Scilit:
- Restriction fragment analysis of the temporal program of bacteriophage SP01 transcription and its control by phage-modified RNA polymerasesVirology, 1977
- Purification of a positive regulatory subunit from phage SP01-modified RNA polymeraseNature, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- Identification of phage SP01 proteins coded by regulatory genes 33 and 34Nature, 1976
- Bacteriophage SP01 regulatory proteins directing late gene transcription in vitroNature, 1976
- The virus-specified subunits of a modified B. subtilis RNA polymerase are determinants of DNA binding and RNA chain initiationCell, 1976
- Regulatory gene 28 of bacteriophage SPO1 codes for a phage-induced subunit of RNA polymeraseJournal of Molecular Biology, 1976
- Highly asymmetric transcription by RNA polymerase containing phage-SP01-induced polypeptides and a new host protein.Proceedings of the National Academy of Sciences, 1975
- Studies of the binding of Escherichia coli RNA polymerase to DNAJournal of Molecular Biology, 1972