Improved procedure for separation and purification of Arthronema africanum phycobiliproteins
Authors:
- 6 January 2007
- journal article
- research article
- Published by Springer Nature in Biotechnology Letters
- Vol. 29 (4) , 647-651
Abstract
A rapid, inexpensive and reliable procedure for separation and purification of C-phycocyanin (C-PC) and allophycocyanin (APC) from Arthronema africanum based on a previously described rivanol-sulfate method for C-PC purification was developed. Exclusion of NaCl from the extraction buffer resulted in complete separation of APC and C-PC, two-fold reduction of rivanol treatments, and a higher yield and purity of C-PC. Pure C-PC (A620/A280 of 4.52) and APC (A652/A280 of 2.41) were obtained. The estimated molecular masses of the α and β subunits were 17 and 19 kDа for С-phycocyanin and 16 and 18 kDа for APC, respectively. The overall C-PC recovery of 55% (w/w) from its content (100 mg) in the crude extract was 10–20% higher than so far reported. The procedure appears promising for scaling up and broader applications.Keywords
This publication has 14 references indexed in Scilit:
- Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitatProtein Expression and Purification, 2005
- Purification of antibody and antibody‐fragment from E. coli homogenate using 6,9‐diamino‐2‐ethoxyacridine lactate as precipitation agentBiotechnology and Bioengineering, 2004
- C-Phycocyanin: A Biliprotein with Antioxidant, Anti-Inflammatory and Neuroprotective EffectsCurrent Protein & Peptide Science, 2003
- Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformisJournal of Biotechnology, 2003
- Purification, Crystallization and Preliminary X-ray Diffraction Studies of C-Phycocyanin and Allophycocyanin fromSpirulina platensisActa Crystallographica Section D Structural Biology, 1997
- Phycobiliproteins in Rhodella reticulata and photoregulatory effects on their contentJournal of Biotechnology, 1996
- Phycobiliproteins — a family of valuable, widely used fluorophoresJournal of Applied Phycology, 1994
- Nonurea Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis with High-Molarity Buffers for the Separation of Proteins and PeptidesAnalytical Biochemistry, 1993
- Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gelsElectrophoresis, 1987
- Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica LemmPlant Physiology, 1955