Effect of D-Galactosamine Administration on Nucleotide and Protein Metabolism in Isolated Rat Kupffer Cells
Authors:
- Friedrich HofmannFHFriedrich Hofmann
- Shreepad R. WagleSWShreepad R. Wagle
- Karl DeckerKDKarl Decker
- 1 January 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (2) , 1395-1400
Abstract
Nucleotide contents of isolated rat Kupffer cells apparently were smaller than those of hepatocytes. Rate of UDPGal [galactose] formation from D-galactose was much lower in Kupffer cells than in hepatocytes. Viability of the former was checked by measuring leakage of enzymes and formation of UTP from uridine. Addition of GalN [D-galactosamine] to isolated Kupffer cells did not decrease their UTP and UDPG contents as much as those of hepatocytes. The same results were obtained when cells were isolated from GalN-pretreated animals. Incorporation of labeled amino acids into protein after GalN addition was much less reduced in Kupffer cells than in hepatocytes. Kupffer cells may not contribute to GalN-induced liver injury as a result of uridylate trapping.Keywords
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