Activity of plasma membrane β‐galactosidase and β‐glucosidase
Authors:
- Massimo AureliMAMassimo Aureli
- Anie Priscilla MasilamaniAMAnie Priscilla Masilamani
- Giuditta IlluzziGIGiuditta Illuzzi
- Nicoletta LobertoNLNicoletta Loberto
- Federica ScandroglioFSFederica Scandroglio
- Alessandro PrinettiAPAlessandro Prinetti
- Vanna ChigornoVCVanna Chigorno
- Sandro SonninoSandro Sonnino
- 3 July 2009
- journal article
- Published by Wiley in FEBS Letters
- Vol. 583 (15) , 2469-2473
Abstract
Human fibroblasts produce ceramide from sialyllactosylceramide on the plasma membranes. Sialidase Neu3 is known to be plasma membrane associated, while only indirect data suggest the plasma membrane association of β‐galactosidase and β‐glucosidase. To determine the presence of β‐galactosidase and β‐glucosidase on plasma membrane, cells were submitted to cell surface biotinylation. Biotinylated proteins were purified by affinity column and analyzed for enzymatic activities on artificial substrates. Both enzyme activities were found associated with the cell surface and were up‐regulated in Neu3 overexpressing cells. These enzymes were capable to act on both artificial and natural substrates without any addition of activator proteins or detergents and displayed a trans activity in living cells.Keywords
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