Fe2O3–SiO2Core–Shell Nanoparticles Conjugated With Bovine Serum Albumin
Authors:
- 15 June 2017
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Magnetics
- Vol. 53 (11) , 1-6
Abstract
This paper presents a systematic study of bovine serum albumin (BSA) conjugation on the amine-functionalized magnetic nanoparticles (NPs) and the effects of various experimental parameters on the conjugation process. The amine-functionalized Fe2O3 NPs with silica shell were synthesized through surface modification. The BSA conjugation on the amine-functionalized Fe2O3 NPs were achieved through covalent binding via carbodiimide activation. Morphology and magnetic properties of the nanoparticles were investigated. The influence of reaction time, the initial protein concentration as well as the temperature on BSA conjugation were studied. The increased reaction time promoted the conjugation of BSA on the Fe2O3 NPs and finally reached the saturation at 43.4 mg/g. The initial BSA concentration can increase the amount of conjugated BSA. The temperature exhibited no significant effect on the conjugation process. The results are useful for designing effective fabrication strategy for protein-conjugated nanomaterials.Keywords
This publication has 34 references indexed in Scilit:
- BSA binding to silica capped gold nanostructures: effect of surface cap and conjugation design on nanostructure–BSA interfaceRSC Advances, 2013
- A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performanceTalanta, 2010
- Single Conducting Polymer Nanowire Chemiresistive Label-Free Immunosensor for Cancer BiomarkerAnalytical Chemistry, 2009
- Applications of Nanoparticles in BiologyAdvanced Materials, 2008
- The present and future of nanotechnology in human health careNanomedicine: Nanotechnology, Biology and Medicine, 2007
- Magnetic properties of polypyrrole-coated iron oxide nanoparticlesJournal of Physics D: Applied Physics, 2005
- Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agentJBIC Journal of Biological Inorganic Chemistry, 2004
- Preparation and characterization of magnetite nanoparticles coated by amino silaneColloids and Surfaces A: Physicochemical and Engineering Aspects, 2003
- Modifying the Surface Properties of Superparamagnetic Iron Oxide Nanoparticles through A Sol−Gel ApproachNano Letters, 2002
- Infrared Spectra of FerritesPhysical Review B, 1955