Use of RNA-Protein Complexes for Genome Editing in Non- albicans Candida Species

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Abstract
Existing CRISPR-Cas9 genome modification systems for use in Candida albicans , which rely on constructs to endogenously express the Cas9 protein and guide RNA, do not work efficiently in other Candida species due to inefficient promoter activity. Here, we present an expression-free method that uses RNA-protein complexes and demonstrate its use in three Candida species known for their drug resistance profiles. We propose that this system will aid the genetic analysis of fungi that lack established genetic systems.
Funding Information
  • HHS | NIH | National Institute of General Medical Sciences (T32GM008704)
  • HHS | NIH | National Institute of General Medical Sciences (R01 GM108492)

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