Identification of Cis-regulatory Elements in the Mouse Pax9/Nkx2-9 Genomic Region: Implication for Evolutionary Conserved Synteny
Authors:
- 1 September 2003
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 165 (1) , 235-242
Abstract
We previously reported close physical linkage between Pax9 and Nkx2-9 in the human, mouse, and pufferfish (Fugu rubripes) genomes. In this study, we analyzed cis-regulatory elements of the two genes by comparative sequencing in the three species and by transgenesis in the mouse. We identified two regions including conserved noncoding sequences that possessed specific enhancer activities for expression of Pax9 in the medial nasal process and of Nkx2-9 in the ventral neural tube. Remarkably, the latter contained the consensus Gli-binding motif. Interestingly, the identified Pax9 cis-regulatory sequences were located in an intron of the neighboring gene Slc25a21. Close examination of an extended genomic interval around Pax9 revealed the presence of strong synteny conservation in the human, mouse, and Fugu genomes. We propose such an intersecting organization of cis-regulatory sequences in multigenic regions as a possible mechanism that maintains evolutionary conserved synteny.Keywords
This publication has 24 references indexed in Scilit:
- Comparative analysis of the genomic organization of Pax9 and its conserved physical association with Nkx2-9 in the human, mouse, and pufferfish genomesMammalian Genome, 2001
- Identification of the Human Mitochondrial Oxodicarboxylate CarrierJournal of Biological Chemistry, 2001
- Human-mouse genome comparisons to locate regulatory sitesNature Genetics, 2000
- MAPK Upstream Kinase (MUK)-binding Inhibitory Protein, a Negative Regulator of MUK/Dual Leucine Zipper-bearing Kinase/Leucine Zipper Protein KinaseJournal of Biological Chemistry, 2000
- PipMaker—A Web Server for Aligning Two Genomic DNA SequencesGenome Research, 2000
- Paradox segmentation along inter- and intrasomitic borderlines is followed by dysmorphology of the axial skeleton in theopen brain (opb) mouse mutantDevelopmental Genetics, 1998
- Pax genes and organogenesisBioEssays, 1997
- Small is beautiful: comparative genomics with the pufferfish (Fugu rubripes)Trends in Genetics, 1996
- Pufferfish and new paradigm for comparative genome analysis.Proceedings of the National Academy of Sciences, 1996
- Enhancer analysis of the mouse HNF‐3β gene: regulatory elements for node/notochord and floor plate are independent and consist of multiple sub‐ elementsGenes to Cells, 1996