Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila
Open Access
- 18 April 2019
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 593 (10) , 1102-1112
Abstract
The Paip2 protein is a factor regulating mRNA translation and stability in the cytoplasm. It has also been found in the nuclei of several cell types in Drosophila. Here, we aim to elucidate the functions of Paip2 in the cell nucleus. We find that nuclear Paip2 is a component of an ~300‐kDa protein complex. Paip2 interacts with mRNA capping factor and factors of RNA polymerase II (Pol II) transcription initiation and early elongation. Paip2 functionally cooperates with the Cbp80 subunit of the cap‐binding complex, with both proteins ensuring proper Pol II C‐terminal domain (CTD) Ser5 phosphorylation at the promoter. Thus, Paip2 is a novel player at the stage of mRNA capping and early Pol II elongation.Keywords
Funding Information
- National Institute of General Medical Sciences (8 P41GM103533, R35 GM126975)
- Russian Science Foundation (16‐14‐10346)
This publication has 51 references indexed in Scilit:
- Suppression of Cellular Transformation by Poly (A) Binding Protein Interacting Protein 2 (Paip2)PLOS ONE, 2011
- Structural Insights to How Mammalian Capping Enzyme Reads the CTD CodeMolecular Cell, 2011
- The poly(A)-binding protein partner Paip2a controls translation during late spermiogenesis in miceJournal of Clinical Investigation, 2010
- The molecular basis for the regulation of the cap-binding complex by the importinsNature Structural & Molecular Biology, 2009
- Use of combined in silico expression data and phylogenetic analysis to identify new oocyte genes encoding RNA binding proteins in the mouseMolecular Reproduction and Development, 2008
- Two Isoforms of Drosophila TRF2 Are Involved in Embryonic Development, Premeiotic Chromatin Condensation, and Proper Differentiation of Germ Cells of Both SexesMolecular and Cellular Biology, 2006
- A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) bindingProceedings of the National Academy of Sciences, 2006
- Poly(A)-binding Protein-interacting Protein 2, a Strong Regulator of Vascular Endothelial Growth Factor mRNAJournal of Biological Chemistry, 2004
- Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2Biochemical Journal, 2003
- Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in VivoScience, 2003