Ki‐67 expression and BrdUrd incorporation as markers of proliferative activity in human prostate tumour models

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Abstract
The validity of the use of the monoclonal antibodies Ki‐67 and anti‐BrdUrd to evaluate proliferative activity of human prostate tumour models was studied. Growth of the transplantable PC‐82 and PC‐EW prostate tumours, as assessed by tumour volume measurements, was significantly correlated with the proliferative activity as reflected by BrdUrd incorporation into DNA (r= 0.64 and r= 0.78, respectively). The proliferative activity of PC‐82 tumours detected by Ki‐67 antigen expression paralleled the pattern observed with BrdUrd (r= 0.51) and a significant correlation (r= 0.60) between the results obtained with both markers was found. In growing PC‐82 and PC‐EW tumours only small variations in the Ki‐67 and BrdUrd indices were observed. In contrast, Ki‐67 expression in regressing PC‐82 tumours varied considerably (2.7 ± 2.2%). The BrdUrd index in regressing PC‐32 tumours showed less variation (1.3 ± 0.2%), but part of the BrdUrd‐positive cells were found in the stromal (murine) part of the regressing tissue. It is concluded that the Ki‐67 and BrdUrd proliferation markers are reliable parameters to monitor changes in growth of prostate tumour lines, but that in slow growing or regressing tumours Ki‐67 and BrdUrd data should be interpreted with caution.

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