Cloning and Characterization of an Intracellular Esterase from the Wine-Associated Lactic Acid BacteriumOenococcus oeni

Authors:
Publisher website
Google Scholar
Abstract
We report the cloning and characterization of EstB28, the first esterase to be so characterized from the wine-associated lactic acid bacterium,Oenococcus oeni. The published sequence forO. oenistrain PSU-1 was used to identify putative esterase genes and design PCR primers in order to amplify the corresponding region from strain Ooeni28, an isolate intended for inoculation of wines. In this way a 912-bp open reading frame (ORF) encoding a putative esterase of 34.5 kDa was obtained. The amino acid sequence indicated that EstB28 is a member of family IV of lipolytic enzymes and contains the GDSAG motif common to other lactic acid bacteria. This ORF was cloned intoEscherichia coliusing an appropriate expression system, and the recombinant esterase was purified. Characterization of EstB28 revealed that the optimum temperature, pH, and ethanol concentration were 40°C, pH 5.0, and 28% (vol/vol), respectively. EstB28 also retained marked activity under conditions relevant to winemaking (10 to 20°C, pH 3.5, 14% [vol/vol] ethanol). Kinetic constants were determined for EstB28 withp-nitrophenyl (pNP)-linked substrates ranging in chain length from C2to C18. EstB28 exhibited greatest specificity for C2to C4pNP-linked substrates.

This publication has 53 references indexed in Scilit: