Genomic characterization of the attenuated human cytomegalovirus strain TR-VAC developed for subviral particle vaccine production
Authors:
Open Access
- 8 October 2026
- journal article
- Published by American Society for Microbiology in Microbiology Resource Announcements
- Vol. 15 (10)
Abstract
We report the complete genome sequence of the attenuated human cytomegalovirus strain TR-VAC, developed for subviral particle vaccine production. Oxford Nanopore duplex sequencing confirmed all engineered modifications, including UL130 repair, UL25 stop codons, ddFKBP insertion, GFP deletion, and retention of the bacterial artificial chromosome backbone, without large-scale structural rearrangements.Keywords
This publication has 9 references indexed in Scilit:
- Genomic and geographical structure of human cytomegalovirusProceedings of the National Academy of Sciences, 2023
- An Attenuated Strain of Human Cytomegalovirus for the Establishment of a Subviral Particle VaccineVaccines, 2022
- Dense Bodies of a gH/gL/UL128/UL130/UL131 Pentamer-Repaired Towne Strain of Human Cytomegalovirus Induce an Enhanced Neutralizing Antibody ResponseJournal of Virology, 2019
- VAPiD: a lightweight cross-platform viral annotation pipeline and identification tool to facilitate virus genome submissions to NCBI GenBankBMC Bioinformatics, 2019
- MUMmer4: A fast and versatile genome alignment systemPLOS Computational Biology, 2018
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009
- The human cytomegalovirusPharmacology & Therapeutics, 2003
- Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic GenesJournal of Virology, 2001
- Candidate cytomegalovirus strain for human vaccinationInfection and Immunity, 1975