Nanopore detection of DNA molecules in magnesium chloride solutions
Open Access
- 20 May 2013
- journal article
- Published by Springer Nature in Discover Nano
- Vol. 8 (1) , 245
Abstract
High translocation speed of a DNA strand through a nanopore is a major bottleneck fornanopore detection of DNA molecules. Here, we choose MgCl2 electrolyte assalt solution to control DNA mobility. Experimental results demonstrate that theduration time for straight state translocation events in 1 M MgCl2solution is about 1.3 ms which is about three times longer than that for thesame DNA in 1 M KCl solution. This is because Mg2+ ions caneffectively reduce the surface charge density of the negative DNA strands and thenlead to the decrease of the DNA electrophoretic speed. It is also found that theMg2+ ions can induce the DNA molecules binding together and reduce theprobability of straight DNA translocation events. The nanopore with small diametercan break off the bound DNA strands and increase the occurrence probability ofstraight DNA translocation events.Keywords
This publication has 34 references indexed in Scilit:
- Transverse electric field dragging of DNA in a nanochannelScientific Reports, 2012
- Slowing down DNA Translocation through a Nanopore in Lithium ChlorideNano Letters, 2012
- Slowing and controlling the translocation of DNA in a solid-state nanoporeNanoscale, 2011
- Characterizing and Controlling the Motion of ssDNA in a Solid-State NanoporeBiophysical Journal, 2011
- Controlled Translocation of Individual DNA Molecules through Protein Nanopores with Engineered Molecular BrakesNano Letters, 2011
- Deciphering ionic current signatures of DNA transport through a nanoporeNanoscale, 2010
- Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradientNature Nanotechnology, 2009
- Electric and electrophoretic inversion of the DNA charge in multivalent electrolytesSoft Matter, 2009
- Reverse DNA translocation through a solid-state nanopore by magnetic tweezersNanotechnology, 2009
- DNA Attraction in Monovalent and Divalent ElectrolytesJournal of the American Chemical Society, 2008