Cabbage Cryoprotectin Is a Member of the Nonspecific Plant Lipid Transfer Protein Gene Family
- 1 February 2001
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 125 (2) , 835-846
Abstract
We have recently purified a protein (cryoprotectin) from the leaves of cold-acclimated cabbage (Brassica oleracea) to electrophoretic homogeneity, which protects thylakoids isolated from the leaves of nonacclimated spinach (Spinacia oleracea) from freeze-thaw damage. Sequencing of cryoprotectin showed the presence of at least three isoforms of WAX9 proteins, which belong to the class of nonspecific lipid transfer proteins. Antibodies raised against two synthetic peptides derived from the WAX9 proteins recognized a band of approximately 10 kD in western blots of crude cryoprotectin preparations. This protein and the cryoprotective activity could be precipitated from solution by the antiserum. We show further that cryoprotectin is structurally and functionally different from WAX9 isolated from the surface wax of cabbage leaves. WAX9 has lipid transfer activity for phosphatidylcholine, but no cryoprotective activity. Cryoprotectin, on the other hand, has cryoprotective, but no lipid transfer activity. The cryoprotective activity of cryoprotectin was strictly dependent on Ca2+ and Mn2+ and could be inhibited by chelating agents, whereas the lipid transfer activity of WAX9 was higher in the presence of ethylenediaminetetraacetate than in the presence of Ca2+and Mn2+.Keywords
This publication has 48 references indexed in Scilit:
- LIPID-TRANSFER PROTEINS IN PLANTSAnnual Review of Plant Biology, 1996
- On beyond classic RACE (rapid amplification of cDNA ends).Genome Research, 1994
- In Vitro Antifungal Activity of a Radish (Raphanus sativus L.) Seed Protein Homologous to Nonspecific Lipid Transfer ProteinsPlant Physiology, 1992
- An abscisic‐acid‐responsive, low temperature barley gene has homology with a maize phospholipid transfer proteinPlant, Cell & Environment, 1992
- Amino acid sequence of a non-specific wheat phospholipid transfer protein and its conformation as revealed by infrared and Raman spectroscopy. Role of disulfide bridges and phospholipids in the stabilization of the α-helix structureBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Use of spin-labeled and fluorescent lipids to study the activity of the phospholipid transfer protein from maize seedlingsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
- Purification and characterization of a non-specific lipid transfer protein from germinated castor bean endosperms which transfers phospholipids and galactolipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Antibodies against individual thylakoid membrane proteins as molecular probes to study chemical and mechanical freezing damage in vitroBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979