3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
- 26 October 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 87 (21) , 10770-10778
Abstract
Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.Keywords
Funding Information
- National Research Foundation of Korea (K20904000004-11A0500-00410)
- Royal Society
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