ONT Post-PCR Pooling & Purification for Fungal Barcoding v2

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Abstract
Overview: The goals of this protocol are to pool subsamples of all your PCR products into a single pooled library tube and to purify that product using magnetic beads. Philosophical note: Equal-volume pooling does not normalize DNA contributions between samples. We do not make an attempt to normalize each PCR reaction concentrations as a part of this protocol. Time required: ~45 minutes (mostly waiting)