Stretching tethered DNA chains in shear flow
Authors:
- P. S DoylePDP. S Doyle
- 1 December 2000
- journal article
- Published by IOP Publishing in Europhysics Letters
- Vol. 52 (5) , 511-517
Abstract
We discuss the stretching oftethered chains subject to a shear flow. Fluores- cence microscopy measurements ofthe extension ofsheared tethered DNA, "model polymers", ofvarious lengths are presented. The tethered chains approach f ull extension as the flow rate is increased, but very slowly. We show that a general theory to understand the large defor- mation ofany sheared tethered chain must include both the correct nonlinear f orce-extension relation and chain fluctuations transverse to the flow direction, which themselves depend on the nonlinear elasticity ofthe chain. Direct comparison ofderived scaling laws to simulations and experiments support our theory.Keywords
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