Peptide nucleic acid probes with charged photocleavable mass markers
Open Access
- 1 July 2010
- journal article
- Published by Taylor & Francis in Artificial DNA: PNA & XNA
- Vol. 1 (1) , 27-35
Abstract
Halogen-labelled peptide organic acid (HPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.Keywords
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