Multiplexed aberration measurement for deep tissue imaging in vivo

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Abstract
An adaptive optics method using multiplexed light measurement and modulation in multiple pupil segments improves structural and functional in vivo imaging over large volumes in strongly scattering mouse brain with only a single aberration correction. We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein–labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

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