Silver nanoparticles synthesized using S. nervosum leaf extract, and their antibacterial and antifungal activity

Authors:
  • PHPham Sy Hieu
    Pham Sy Hieu
  • NNNguyen Hong Nhung
    Nguyen Hong Nhung
  • TCTran Que Chi
    Tran Que Chi
  • THTran Thi Huong
    Tran Thi Huong
  • PKPham Duy Khanh
    Pham Duy Khanh
  • PGPham Thi Gam
    Pham Thi Gam
  • NPNguyen Van Phuong
    Nguyen Van Phuong
  • PNPhan Thi Thanh Nga
    Phan Thi Thanh Nga
  • HYHoang Hien Y
    Hoang Hien Y
  • DUDao My Uyen
    Dao My Uyen
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Abstract
In this study, the green synthesis of silver nanoparticles (AgNPs) was performed using S.nervosum leaf extract, and their physicochemical characterization was also studied. The characteristic surface plasmon resonance peak observed in the visible region at 435 nm is consistent with previous reports of silver nanoparticle formation. The synthesized AgNPs were also characterized by SEM, TEM, XRD, and FTIR, which revealed their nearly spherical shape, with a diameter mainly in the 10 - 30 nm range. The antimicrobial activity of AgNPs was studied using the agar well diffusion method on four clinically significant pathogenic microorganisms (P. aeruginosa ATCC 27853; E. coli ATCC 35218, S. aureus ATCC 12493; S. aureus Mastitis isolate). The results showed that the green-synthesized AgNPs exhibited antimicrobial activity at most studied concentrations, including the antibacterial effect against the S. Aureus (Mastitis isolate) strain, which was found to be resistant to Tetracycline at 100 µg/L. The antifungal activity of these nanoparticles was also studied on Colletotrichum gloeopsoriodes by colony formation assay. The green synthesis of AgNPs was successfully conducted for the first time using the aqueous extract of S. nervosum. The results of this study indicate that the silver nanoparticles have potential applications as antibacterial and antifungal agents.