Periodontal wound healing/regeneration following the application of rhGDF‐5 in a β‐TCP/PLGA carrier in critical‐size supra‐alveolar periodontal defects in dogs

Authors:
Publisher website
Google Scholar
Abstract
Kwon DH, Bisch FC, Herold RW, Pompe C, Bastone P, Rodriguez NA, Susin C, Wikesjö UM. Periodontal wound healing/regeneration following application of rhGDF‐5 in a β‐TCP/PLGA carrier in critical‐size supra‐alveolar periodontal defects in dogs. J Clin Periodontol 2010; 37: 667–674. doi: 10.1111/j.1600‐051X.2010.01569.x. Aim: The objective of this study was to evaluate the effect of a novel recombinant human GDF‐5 (rhGDF‐5) construct intended for onlay and inlay indications on periodontal wound healing/regeneration.Methods: Contralateral, surgically created, critical‐size, 6‐mm, supra‐alveolar periodontal defects in five adult Hound Labrador mongrel dogs received rhGDF‐5 coated onto β‐tricalcium phosphate (β‐TCP) particles and immersed in a bioresorbable poly(lactic‐co‐glycolic acid) (PLGA) composite or the β‐TCP/PLGA carrier alone (control). The rhGDF‐5 and control constructs were moulded around the teeth and allowed to set. The gingival flaps were then advanced; flap margins were adapted 3–4 mm coronal to the teeth and sutured. The animals were euthanized at 8 weeks post‐surgery when block biopsies were collected for histometric analysis.Results: Healing was generally uneventful. A few sites exhibited minor exposures. Three control sites and one rhGDF‐5 site (in separate animals) experienced more extensive wound dehiscencies. The rhGDF‐5 and control constructs were easy to apply and exhibited adequate structural integrity to support the mucoperiosteal flaps in this challenging onlay model. Limited residual β‐TCP particles were observed at 8 weeks for both rhGDF‐5/β‐TCP/PLGA and β‐TCP/PLGA control sites. The rhGDF‐5/β‐TCP/PLGA sites showed significantly greater cementum (2.34 ± 0.44 versus 1.13 ± 0.25 mm, p=0.02) and bone (2.92 ± 0.66 versus 1.21 ± 0.30 mm, p=0.02) formation compared with the carrier control. Limited ankylosis was observed in four of five rhGDF‐5/β‐TCP/PLGA sites but not in control sites.Conclusions: Within the limitations of this study, the results suggest that rhGDF‐5 is a promising candidate technology in support of periodontal wound healing/regeneration. Carrier and rhGDF‐5 dose optimization are necessary before further advancement of the technology towards clinical evaluation.

This publication has 50 references indexed in Scilit: