Production of Glutaconic Acid in a RecombinantEscherichia coliStrain
Authors:
- 1 January 2011
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (1) , 320-322
Abstract
The assembly of six genes that encode enzymes from glutamate-fermenting bacteria convertedEscherichia coliinto a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutaryl-coenzyme A (CoA), and (E)-glutaconyl-CoA to yield 2.7 ± 0.2 mM (E)-glutaconate in the medium.Keywords
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