Topology and Function of CcmD in Cytochrome c Maturation
Authors:
- 15 May 2008
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 190 (10) , 3489-3493
Abstract
The system I cytochrome c biogenesis pathway requires CcmD, a small polypeptide of 69 residues in Escherichia coli . Here it is shown that CcmD is a component of the CcmABC ATP-binding cassette transporter complex. CcmD is not necessary for the CcmC-dependent transfer of heme to CcmE in the periplasm or for interaction of CcmE with CcmABC. CcmD is absolutely required for the release of holo-CcmE from the CcmABCD complex. Evidence is presented that the topology of CcmD in the cytoplasmic membrane is the N terminus outside and the C terminus inside with one transmembrane domain.Keywords
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