Versatility of Enzymes Catalyzing Late Steps in Polyene 67-121C Biosynthesis
Authors:
- 23 April 2013
- journal article
- research article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 77 (4) , 880-883
Abstract
Actinoplanes caeruleus produces 67-121C, a heptaene macrolide modified with a D-mannosyl-D-mycosaminyl disaccharide. Draft genome sequencing revealed genes encoding mycosaminyltransferase, mycosamine synthase, a cytochrome P450 that modifies the macrolactone core, and the extending mannosyltransferase. Only the mycosamine synthase and P450 were active in the biosynthesis of amphotericins in Streptomyces nodosus, the amphotericin producer.Keywords
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