Tritium sequence analysis of oligoribonucleotides: a combination of post-labeling and thin-layer chromatographic techniques for the analysis of partial snake venom phosphodiesterase digests
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Open Access
- 1 October 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (10) , 1329-1342
Abstract
A tritium derivative method for sequence analysis of poly-ribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide-3′ dialdehydes generated by controlled snake venom phosphodiesterase/alkaline phosphomonoesterase digestion and periodate treatment of time point aliquots of the incubation mixture. Radioactive oligonucleotide derivatives are resolved according to chain length by PEI-cellulose 1 anion-exchange TLC and their 3′-termini identified by techniques described in the preceding paper of this series 2 . The present tritium derivative method is compared with the one described previously 2 .Keywords
This publication has 20 references indexed in Scilit:
- DNA Sequencing TechniquesAnnual Review of Biochemistry, 1974
- Tritium sequence analysis of polyribonucleotides following periodate‐phosphomonoesterase degradation — characterization of oligonucleotide‐3′ dialdehyde intermediatesFEBS Letters, 1974
- Derivatives ofN-[N-(9-β-D-ribofuranosylpurin-6-yl)carbamoyl]threonine in phosphodiesterase hydrolysates of wheat embryo transfer ribonucleic acidBiochemistry, 1974
- Continuous directional degradation — a novel method for sequence analysis of polyribonucleotidesFEBS Letters, 1973
- The corrected nucleotide sequence of yeast leucine transfer ribonucleic acidBiochemical and Biophysical Research Communications, 1973
- Primary sequence of glutamic acid tRNA II fromEscherichia coliFEBS Letters, 1972
- Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from YeastScience, 1971
- An evaluation of film detection methods for weak β-emitters, particularly tritiumAnalytical Biochemistry, 1970
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965
- The detection of terminal nucleoside residues in polyribonucleotidesBiochimica et Biophysica Acta, 1960