Alterations in number of protofilaments in microtubules assembled in vitro.

Authors:
  • GPG B Pierson
    G B Pierson
  • PBP R Burton
    P R Burton
  • RHR H Himes
    R H Himes

Abstract
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold-dissociation followed by reassembly in vitro.

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