Synthesis of RNA Using 2‘-O-DTM Protection
- 31 August 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (38) , 12356-12357
Abstract
tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2‘-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2‘-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5‘-O-DMTr function can be used for high-throughput cartridge RNA purification.Keywords
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