Synthesis and Labeling of RNA In Vitro
Authors:
Open Access
- 1 April 2013
- journal article
- unit
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 102 (1) , 4.15.1-4.15.14
Abstract
This unit discusses several methods for generating large amounts of uniformly labeled, end‐labeled, and site‐specifically labeled RNAs in vitro. The methods involve a number of experimental procedures, including RNA transcription, 5′ dephosphorylation and rephosphorylation, 3′ terminal nucleotide addition (via ligation), site‐specific RNase H cleavage directed by 2′‐O‐methyl RNA‐DNA chimeras, and 2‐piece splint ligation. The applications of these RNA radiolabeling approaches are also discussed. Curr. Protoc. Mol. Biol. 102:4.15.1–4.15.14. © 2013 by John Wiley & Sons, Inc.Keywords
This publication has 15 references indexed in Scilit:
- Synthesis of Peptide‐Oligonucleotide Conjugates Using a Heterobifunctional CrosslinkerCurrent Protocols in Nucleic Acid Chemistry, 2010
- Targeted 2′-O Methylation at a Nucleotide within the Pseudoknot of Telomerase RNA Reduces Telomerase Activity In VivoMolecular and Cellular Biology, 2010
- Nucleotide Analog Interference Mapping2009
- Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes2006
- Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA‐guided or RNA‐independent mechanismThe EMBO Journal, 2005
- Detection and quantitation of RNA base modificationsRNA, 2004
- Pseudouridylation (Ψ) of U2 snRNA in S.cerevisiae is catalyzed by an RNA‐independent mechanismThe EMBO Journal, 2003
- Construction of 4-Thiouridine Site-Specifically Substituted RNAs for Cross-Linking StudiesMethods, 1999
- Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesNucleic Acids Research, 1987
- A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structureNucleic Acids Research, 1985