A New Hematocrit Measurement Method Using a Chemiluminescence Biosensor and Its Application in a Chemiluminescence Immunoassay Platform for Myocardial Markers Detection with Whole Blood Samples

Authors:
Open Access
Publisher website
Google Scholar
Abstract
The accuracy and precision of analyte concentrations measured in whole blood by chemiluminescence immunoassay (CLIA) have been significantly affected by erythrocytes, which leads to poor application of whole blood CLIA in clinical practice. In this work, a chemiluminescence biosensing optical platform for blood hematocrit (HCT) analysis using MAGICL 6000 (Getein Biotechnology, Nanjing, China) was designed, implemented, and fully characterized. The developed method was successfully applied to determine various HCT levels of human blood from 0% to 65%, with a correlation coefficient of 0.9885 compared with the conventional method (Sysmex XE 5000, Kobe, Japan). A mathematical model was developed to quantitatively evaluate the impact of HCT on the results of two sample types (whole blood vs. plasma). Combining the established HCT method and mathematical model with CLIA on MAGICL 6000, the precision was significantly improved by almost 20%. Comparison studies using whole blood samples and corresponding plasma samples showed that the square of the correlation coefficients of troponin I (cTnI), myoglobin (MYO), creatine kinase MB (CK-MB), and N-terminal pro-hormone brain natriuretic peptide (NT-proBNP) were increased to 0.9992, 0.9997, 0.9996, and 0.9994, respectively, showing a great potential for clinical application.
Funding Information
  • Jiangsu Provincial Special Program of Medical Science (BE2019749, 62071119, BA2020016, SKLOD2022OF05)
  • National Natural Science Foundation of China (BE2019749, 62071119, BA2020016, SKLOD2022OF05)
  • Jiangsu Provincial Key Research and Development Program (BE2019749, 62071119, BA2020016, SKLOD2022OF05)
  • Open Funding of State Key Laboratory of Oral Diseases (BE2019749, 62071119, BA2020016, SKLOD2022OF05)

This publication has 54 references indexed in Scilit: