Rapid, single-step purification of restriction endonucleases on Cibacron Blue F3GA-agarose

Authors:
  • KBKrishna Baksi
    Krishna Baksi
  • DRDavid L. Rogerson
    David L. Rogerson
  • GRGeorge W. Rushizky
    George W. Rushizky
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Abstract
After sonication and high-speed centrifugation, crude extracts of B. amyloliquefaciens, P. alcalifaciens, X. holicola, and B. globiggi were adsorbed on the dye Cibacron blue F3GA convalently cross-linked to agarose. The restriction endonucleases BamHI, PalI, XhoI, and BglI together with BglII were isolated by elution of the dye column with linear gradients to 0.5 M NaCl. The enzymes so purified were free of contaminating nucleic acids and other nucleases and were sufficiently concentrated for direct, specific DNA hydrolysis.