Multimodal microscopy with sub-30 fs Yb fiber laser oscillator
Open Access
- 27 June 2012
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 3 (7) , 1750-1756
Abstract
Nonlinear optical microscopy with sub-30 fs pulses from an Yb-fiber laser, approximately three times shorter than typical fiber laser pulses, leads to an order of magnitude brighter third harmonic generation imaging. Multiphoton fluorescence, second and third harmonic generation modalities are compared on stained microspheres and unstained biological tissues.Keywords
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