Characterization of Human Chorionic Gonadotropin and Luteinizing Hormone Contained in Urinary Extracts by Polyacrylamide Gel Electrophoresis

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Abstract
Urinary human chorionic gonadotropin (hCG) and urinary human luteinizing hormone (hLH) contained in the same extract were identified and characterized in terms of molecular size and net charge, using polyacrylamide gel electrophoresis (PAGE) in conjunction with radioimmunoassay. The molecular sizes, expressed as retardation coefficients (KR), in PAGE (pH 10.2, 0 C) were: 0.05 for immunoreactive LH from normal “Albert fraction B” (F×B) : 0.12 and 0.16 for F×B from choriocarcinoma patients with high titers and for commercial hCG; and 0.08 for mixtures of commercial hCG with F×B from patients with gonadotropin titers of 500 IU/24 hours or less.