Impact of glycomacropeptide on growth, adhesion, and biofilm formation of the probiotic Lacticaseibacillus rhamnosus GG

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Abstract
Glycomacropeptide (GMP) is a milk-derived bioactive peptide with demonstrated prebiotic properties. It is composed of a 64-amino acid framework and different carbohydrate molecules, which may serve as carbon sources for beneficial bacteria. Lacticaseibacillus rhamnosus GG (LGG) is a widely used probiotic strain that promotes intestinal barrier function, prevents pathogen colonisation and exerts anti-inflammatory and immunomodulatory activities. In this study, we explored the effect of GMP supplementation to LGG cultures on growth, adhesion and biofilm-forming properties of the bacterium. Prebiotic GMP promoted LGG growth and acidifying activities, mainly under anaerobiosis, without modifying the binding-mucus ability. GMP supplementation also increased LGG-biofilm biomass 3.8- and 3.4-fold under aerobic and anaerobic conditions. Besides, GMP treatment increased LGG-biofilm volume 4.7-fold, thickness by 39% and roughness by 110%. Thus, incorporating GMP into food or nutraceutical formulations might represent a viable strategy to enhance the probiotic efficacy of LGG strain, potentially improving its performance in functional products.
Funding Information
  • Universidad Autónoma de Aguascalientes (PIBB20-1)
  • Instituto para el Desarrollo de la Sociedad del Conocimiento del Estado de Aguascalientes
  • Consejo Nacional de Humanidades, Ciencias y Tecnologías (602709)

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