Analytical‐Band Centrifugation of an Active Enzyme‐Substrate Complex
Authors:
- Michel MireMMMichel Mire
- 1 November 1971
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 23 (2) , 267-275
Abstract
The analytical active‐enzyme‐centrifugation method permits the determination of the hydro‐dynamic properties of enzymes while they are fully active on thier substrates; the centrifugation can be performed at the very dilute concentrations used in kinetic studies (∼ 1 μg/ml); further‐more a purified enzyme preparation is not necessary and in many cases a crude extract is sufficient. This method is beginning to be used in several laboratories even though few of its experimental aspects have been published. This paper reports the details for carrying out an active‐enzyme‐centrifugation run, discusses the experimental conditions and deals with artifacts and ways, which are easy, to overcome them.Keywords
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