Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins
Authors:
- 20 May 2013
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Nanoscale
- Vol. 5 (12) , 5303-5306
Abstract
Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.Keywords
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