Characterisation and determination of indole alkaloids in frog‐skin secretions by electrospray ionisation ion trap mass spectrometry
- 29 January 2002
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (5) , 346-354
Abstract
The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5‐hydroxytryptamine (5‐HT), N ′‐methyl 5‐hydroxytryptamine ( N ′‐methyl 5‐HT), N ′, N ′‐dimethyl 5‐hydroxytryptamine (bufotenine), N ′, N ′, N ′‐trimethyl 5‐hydroxytryptamine (5‐HTQ), and N ′, N ′‐dimethyl 5‐methoxytryptamine (5‐MeODMT) are presented with proposed structures given for each product ion observed. Such MS n experiments can be used to differentiate the isobaric molecular ions of the compounds 5‐HTQ (M + ) and 5‐MeODMT (MH + ). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea , by LC/ESI‐ion trap MS is also presented. The concentrations of 5‐HT, N ′‐methyl 5‐HT and 5‐HTQ were found to be 2.68, 0.26 and 0.54 µg per mg of skin secretion, respectively. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
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