The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth

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Abstract
Currently, a clear interest has been given to berries due to their richness in active metabolites, including anthocyanins and non-coloured phenolics. Therefore, the main aim of the present work is to investigate the phenolic profile, antioxidant abilities, and antiproliferative effects on normal human dermal fibroblasts (NHDF) and human colon carcinoma cell line (Caco-2) cells of phenolic-rich extracts from three red fruits highly appreciated by consumers: two species of blackberries (Rubus fruticosus and Rubus ulmifolius) and one species of mulberry (Morus nigra). A total of 19 different phenolics were identified and quantified by HPLC-DAD-ESI/MSn and HPLC-DAD, respectively. Focusing on the biological potential of the phenolic-rich extracts, all of them revealed notable scavenging abilities. Concerning the antiproliferative properties, R. fruticosus presented a cytotoxic selectivity for Caco-2 cells compared to NHDF cells. To deeper explore the biological potential, combinations with positive controls (ascorbic acid and 5-fluorouracil) were also conducted. Finally, the obtained data are another piece of evidence that the combination of phenolic-rich extracts from natural plants with positive controls may reduce clinical therapy costs and the possible toxicity of chemical drugs.
Funding Information
  • Foundation for Science and Technology (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • Ministry of Science, Technology and Higher Education (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • European Social Fund (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • Fundação para a Ciência e a Tecnologia (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • CICS-UBI (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • Community Funds (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • RedFruit4Health (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • PharmaStar (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)
  • European Union’s Horizon 2020 Research and Innovation Programme (2020.04947.BD, UIDP/00709/2020, UIDB/00709/2020, CENTRO-04-3559-FSE-000162, PRR-C05-i03-I-000143, PD21-00023, 101034371)

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