Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores
Authors:
Open Access
- 30 June 2017
- journal article
- research article
- Published by Springer Nature in Scientific Reports
- Vol. 7 (1) , 1-10
Abstract
This paper presents eigen-analysis of image stack of blinking fluorophores to identify the components that enable super-resolved imaging of blinking fluorophores. Eigen-analysis reveals that the contributions of spatial distribution of fluorophores and their temporal photon emission characteristics can be completely separated. While cross-emitter cross-pixel information of spatial distribution that permits super-resolution is encoded in two matrices, temporal statistics weigh the contribution of these matrices to the measured data. The properties and conditions of exploitation of these matrices are investigated. Con-temporary super-resolution imaging methods that use blinking for super-resolution are studied in the context of the presented analysis. Besides providing insight into the capabilities and limitations of existing super-resolution methods, the analysis shall help in designing better super-resolution techniques that directly exploit these matrices.Keywords
This publication has 31 references indexed in Scilit:
- Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution ImagingAnnual Review of Physical Chemistry, 2012
- Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)Optical Nanoscopy, 2012
- Bayesian localization microscopy reveals nanoscale podosome dynamicsNature Methods, 2011
- Evaluation of fluorophores for optimal performance in localization-based super-resolution imagingNature Methods, 2011
- Fast, single-molecule localization that achieves theoretically minimum uncertaintyNature Methods, 2010
- Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)Proceedings of the National Academy of Sciences, 2009
- Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization MicroscopyBiophysical Journal, 2006
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Nature Methods, 2006
- Precise Nanometer Localization Analysis for Individual Fluorescent ProbesBiophysical Journal, 2002