A spontaneous genetically-induced epiallele at a retrotransposon shapes host genome function
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Abstract
Intracisternal A-particles (IAPs) are endogenous retroviruses (ERVs) responsible for most insertional mutations in the mouse. Full-length IAPs harbour genes flanked by long terminal repeats (LTRs). Here, we identify a solo LTR IAP variant ( C57iap1 solo ) recently formed in the inbred C57BL/6J mouse strain. In contrast to the C57BL/6J full-length IAP at this locus ( C57iap1 full ), C57iap1 solo lacks DNA methylation and H3K9 trimethylation. The distinct DNA methylation levels between the two alleles are established during preimplantation development, likely due to loss of KRAB zinc finger protein binding at the C57iap1 solo variant. C57iap1 solo methylation increases and becomes more variable in a hybrid genetic background yet is unresponsive to maternal dietary methyl supplementation. Differential epigenetic modification of the two variants is associated with metabolic differences and tissue-specific changes in adjacent gene expression. Our characterisation of C57iap1 as a genetically-induced epiallele with functional consequences establishes a new model to study transposable element repression and host-element co-evolution.Keywords
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- Published version: eLife, 10.
This publication has 55 references indexed in Scilit:
- KRAB zinc finger protein diversification drives mammalian interindividual methylation variabilityProceedings of the National Academy of Sciences, 2020
- Metastable epialleles and their contribution to epigenetic inheritance in mammalsSeminars in Cell & Developmental Biology, 2020
- The DNA methyltransferase DNMT3C protects male germ cells from transposon activityScience, 2016
- Regulatory evolution of innate immunity through co-option of endogenous retrovirusesScience, 2016
- Mutations in hereditary phosphoglucomutase 1 deficiency map to key regions of enzyme structure and functionJournal of Inherited Metabolic Disease, 2014
- Developmentally Regulated piRNA Clusters Implicate MILI in Transposon ControlScience, 2007
- MIWI2 Is Essential for Spermatogenesis and Repression of Transposons in the Mouse Male GermlineDevelopmental Cell, 2007
- Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3LNature, 2004
- Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts.Genes & Development, 1994
- Epigenetic control of transgene expression and imprinting by genotype-specific modifiersCell, 1990