Authentication Analysis of MT-4 Cells Distributed by the National Institutes of Health AIDS Reagent Program
- 1 December 2019
- journal article
- editorial
- Published by American Society for Microbiology in Journal of Virology
- Vol. 93 (24)
Abstract
The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. MT-4 cells (lot 150048), distributed by the NIH AIDS Reagent Program (NIH-ARP), were found to be Tax deficient and unable to host replication of the gp41-truncated HIV-1 mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide MT-4 cells.Keywords
This publication has 23 references indexed in Scilit:
- Global Rescue of Defects in HIV-1 Envelope Glycoprotein Incorporation: Implications for Matrix StructurePLOS Pathogens, 2013
- Second-Site Compensatory Mutations of HIV-1 Capsid MutationsJournal of Virology, 2011
- 2′-Deoxy-2′-spirocyclopropylcytidine Revisited: A New and Selective Inhibitor of the Hepatitis C Virus NS5B PolymeraseJournal of Medicinal Chemistry, 2010
- Moderate Influence of Human APOBEC3F on HIV-1 Replication in Primary LymphocytesJournal of Virology, 2010
- Cell line cross‐contamination initiative: An interactive reference database of STR profiles covering common cancer cell linesInternational Journal of Cancer, 2009
- Evidence that Productive Human Immunodeficiency Virus Type 1 Assembly Can Occur in an Intracellular CompartmentJournal of Virology, 2009
- Identification and Characterization of HIV‐1 CD8+T Cell Escape Variants with Impaired FitnessThe Journal of Infectious Diseases, 2008
- Viral RNA Is Required for the Association of APOBEC3G with Human Immunodeficiency Virus Type 1 Nucleoprotein ComplexesJournal of Virology, 2005
- Persistent infection of MT-4 cells by human immunodeficiency virus type 1 becomes increasingly likely with in vitro serial passage of wild-type but not nef mutant virusJournal of General Virology, 1994
- Infection of HTLV-III/LAV in HTLV-I-Carrying Cells MT-2 and MT-4 and Application in a Plaque AssayScience, 1985