Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis
Authors:
- 2 March 2022
- book chapter
- Published by Springer Nature
- Vol. 2419, 169-182
Abstract
Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes. Key words Atherosclerosis Endothelial cell activation Inflammation Monocytes Monocyte adhesion assay Adhesion moleculesKeywords
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